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KMID : 1145120190170010139
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Asian Journal of Beauty and Cosmetology
2019 Volume.17 No. 1 p.139 ~ p.147
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Inhibitory Effects of Prunin on Photo-aging in Human Keratinocytes (HaCaT) Damaged by UVB Radiation
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Ryu Ji-Young
Rhie Sung-Ja
Lim Kye-Hwa
Choi Young-Eun
Han Hyo-Sun
Yang Hyun-Ok
Na Eun-Ju
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Abstract
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Purpose: The aim of this study was to investigate the effects of prunin on photoaging in human keratinocytes (HaCaT) damaged by UVB radiation, so as to find out whether prunin can be used as an effective anti-aging ingredient in cosmetic products.
Method: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to identify the increased expression of MMP1 mRNA and decreased expression of COL1A1 mRNA due to UVB, and luciferase reporter assay was used to determine whether the change in MMP1 mRNA is related to AP-1 transcriptional activity. Furthermore, ELISA analysis was used to determine the change in type I procollagen production.
Results: Prunin protected cells from UVB-induced cytotoxicity, and the increased expression of MMP1 mRNA due to oxidative stress in UVB-damaged HaCaT decreased in a concentration-dependent manner. AP-1 transcriptional activity also decreased in a concentration-dependent manner. The expression of Type I procollagen and COL1A1 mRNA increased in a concentration-dependent manner.
Conclusion: This study found that prunin has an inhibitory efficacy on photoaging due to UV radiation, which is a leading cause of oxidative stress. Thus prunin can be used as an effective cosmetic raw material for inhibiting photoaging. However, it will be necessary to conduct experiments on the human body to find a practical way of applying prunin as a cosmetic raw material for the skin.
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KEYWORD
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Prunin, Photo-aging, Human keratinocyte, MMP1, Ultraviolet B
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